“ Special Crop Science Lab ”

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List of frequently used sites in the lab

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작성일 2024-10-25

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-Search for papers and explore information

BRIC (https://www.ibric.org/bric/index.do )
Pubmed (https://pubmed.ncbi.nlm.nih.gov/ )
Google scholar (https://scholar.google.co.kr/schhp?hl=ko )
Sci-hub (https://www.sci-hub.se/ )
Journal Citation Reports (https://jcr.clarivate.com/jcr/home?Init=Yes&SrcApp=IC2LS )



-Genome database

ePlant (https://bar.utoronto.ca/ )
Phytozome (https://phytozome-next.jgi.doe.gov/blast-search )
Ensembl Plants (https://plants.ensembl.org/index.html )



-DNA/protein sequence

Multalin interface (http://multalin.toulouse.inra.fr/multalin/ ):  sequence alignment
EMBOSS Needle (https://www.ebi.ac.uk/jdispatcher/psa/emboss_needle ): sequence alignment
Reverse Complement (https://www.bioinformatics.org/sms/rev_comp.html ): converts a DNA sequence into its reverse



-PCR primer, SgRNA

NEB Tm Calculator (https://tmcalculator.neb.com/#!/main ): estimate an appropriate annealing temperature
NEBioCalculator (https://nebiocalculator.neb.com/#!/ligation ): calculate the mass of insert required at several molar insert:vector ratios
CRISPR RGEN Tools (http://www.rgenome.net/cas-designer/ ): guide-RNA designer for CRISPR/Cas