Efficient in vitro plant regeneration from hybrid rhizomes of Cymbidium sinense seeds
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Journal. Horticulture, Environment, and Biothchnology. 52 (3):303-308.
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Abstract
An oriental orchid that has leaves striped with different colors is considered to be one of the valuable hybrid cultivars. To propagate this hybrid cultivar, C. sinense Willd Makino (Kumwhasan, Bosei) and C. sinense Willd Makino (Seobo) were crossed. The crossed seeds were cultivated in vitro to induce rhizome development since rhizome-mediated propagation can shorten the long-term (by at least 6 years) propagation time and overcome variations induced by seedling propagation. Knudson and/or Hyponex medium with or without natural products were used for the rhizome and shoot induction. The optimum concentration of activated charcoal (AC) was 0.025% for the best shoot induction rate in line No. 115-1 where 1 mg · L−1 of NAA and 0.5 mg · L−1 of BA were added to the Hyponex medium and in line No. 115-2 where only natural products were added to the Knudson plus Hyponex medium. However, 0.2% of AC was shown to be optimal for line No. 118, which was grown on Hyponex medium without plant growth regulators.
An oriental orchid that has leaves striped with different colors is considered to be one of the valuable hybrid cultivars. To propagate this hybrid cultivar, C. sinense Willd Makino (Kumwhasan, Bosei) and C. sinense Willd Makino (Seobo) were crossed. The crossed seeds were cultivated in vitro to induce rhizome development since rhizome-mediated propagation can shorten the long-term (by at least 6 years) propagation time and overcome variations induced by seedling propagation. Knudson and/or Hyponex medium with or without natural products were used for the rhizome and shoot induction. The optimum concentration of activated charcoal (AC) was 0.025% for the best shoot induction rate in line No. 115-1 where 1 mg · L−1 of NAA and 0.5 mg · L−1 of BA were added to the Hyponex medium and in line No. 115-2 where only natural products were added to the Knudson plus Hyponex medium. However, 0.2% of AC was shown to be optimal for line No. 118, which was grown on Hyponex medium without plant growth regulators.