전남대학교 특용작물학실험실, Chonnam national university Special Crop Science Lab

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> The differential transcript patterns of five antioxidant genes, four genes related to the ginsenoside pathway and five P450 genes related to defense mechanism were investigated in in vitro adventitious roots of Panax ginseng after exposure to two different concentrations of heavy metals for 7 days. PgSOD-1 and PgCAT transcription increased in a dose-dependent manner during the exposure to CuCl2, NiCl2, and CdCl2, while all other tested scavenging enzymes didn’t show significant increase during heavy metal exposure. Conversely, the mRNA transcripts of PgSQE, PgDDS were highly responsive to CuCl2 compared to NiCl2 exposure. However, the transcript profile of Pgβ-AS was highly induced upon NiCl2 treatment compared to CuCl2 and CdCl2 exposure. The expressions of PgCYP716A42, PgCYP71A50U, and PgCYP82C22 were regulated in similar manners, and all showed the highest transcript profile at 100 μM of CuCl2, CdCl2, and NiCl2 except PgCYP71D184, which showed the highest transcript level when subjected to 10 μM CuCl2 and NiCl2. Thus it may suggest that in P. ginseng heavy metal interaction on cell membrane induced expression of various defense related genes via jasmonic acid pathway and also possesses cross talk networks with other defense related pathways.
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“ Special Crop Science Lab ”

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